GeneTex
United States (US)

RPS6 (phospho Ser235) antibody [9H270]

Cat No. GTX15031

Host Rabbit
Clonality Monoclonal
Clone Name 9H270
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat, Xenopus laevis
APPLICATION

Application Note

WB: Use at a dilution of 1/5000 - 1/10000. This dilution of antibody detects RPS6 in serum stimulated rat 3T3 cells. Proteins are visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

29 kDa. ( Note )

Positive Control

Serum stimulated rat 3T3 cells
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide containing the sequence ...RRL[pS]SL... in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S6,S6,Rps6

Cellular Localization

Cytoplasmic

Background

40S ribosomal protein S6 (also known as RPS6) is a ~31 kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247, due to the positions of these amino acid residues on the alpha-helix. Hyperphosphorylation of RPS6 stimulates protein synthesis that mediates progression through the cell cycle.

Database

Research Area

Package List Price ($)
$ 299