APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T (starvation 16hr add 100 nM Rapamycin 30min) , 293T (starvation 16hr stimulate with 100nM Insulin/medium 30min) , 293T (starvation 16hr add 100 nM Rapamycin 30min then stimulate with 100nM Insulin 30min)
Predict Reactivity
Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser235 of human RPS6. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886596
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S6 , S6
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX132281 WB Image
Untreated (–) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235) antibody (GTX132281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132281 IHC-P Image
RPS6 (phospho Ser235) antibody detects RPS6 (phospho Ser235) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPS6 (phospho Ser235) antibody (GTX132281) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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