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RPS6 (phospho Ser235) antibody

Anti-RPS6 (phospho Ser235) antibody used in Western Blot (WB). GTX132281
Anti-RPS6 (phospho Ser235) antibody used in IHC (Paraffin sections) (IHC-P). GTX132281

Cat. No. GTX132281

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T (starvation 16hr add 100 nM Rapamycin 30min) , 293T (starvation 16hr stimulate with 100nM Insulin/medium 30min) , 293T (starvation 16hr add 100 nM Rapamycin 30min then stimulate with 100nM Insulin 30min)

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser235 of human RPS6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886596

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 , S6

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPS6 (phospho Ser235) antibody used in Western Blot (WB). GTX132281

GTX132281 WB Image

Untreated (–) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 (phospho Ser235) antibody (GTX132281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS6 (phospho Ser235) antibody used in IHC (Paraffin sections) (IHC-P). GTX132281

GTX132281 IHC-P Image

RPS6 (phospho Ser235) antibody detects RPS6 (phospho Ser235) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPS6 (phospho Ser235) antibody (GTX132281) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for RPS6 (phospho Ser235) antibody (GTX132281). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169