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RPS6 (phospho Ser240/Ser244) antibody

Anti-RPS6 (phospho Ser240/Ser244) antibody used in Immunoprecipitation (IP). GTX66604
Anti-RPS6 (phospho Ser240/Ser244) antibody used in Western Blot (WB). GTX66604

Cat No. GTX66604

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IP 1:50-1:100
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S240/244 of human RPS6KB1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein S6 , S6

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPS6 (phospho Ser240/Ser244) antibody used in Immunoprecipitation (IP). GTX66604

GTX66604 IP Image

IP analysis of 293T cells treated by PMA/TPA (200 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX66604 RPS6 (phospho Ser240/Ser244) antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-RPS6 (phospho Ser240/Ser244) antibody used in Western Blot (WB). GTX66604

GTX66604 WB Image

WB analysis of HeLa cells treated by 10% FBS at 37ºC for 30 minutes and NIH/3T3 cells treated by 10% FBS at 37ºC for 30 minutes after serum-starvation overnight using GTX66604 RPS6 (phospho Ser240/Ser244) antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

There are currently no references for RPS6 (phospho Ser240/Ser244) antibody (GTX66604). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329