RPS6 (phospho Ser240/Ser244) antibody

Cat. No. GTX66604

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human, Mouse
Package	100 μl ($399)

See all RPS6 products

Datasheet
Document

View product citations for antibody GTX66604 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IP	1:50 - 1:100

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S240/244 of human RPS6KB1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 , S6

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6194 RPS6
UniProt: P62753 RPS6

Research Area

DATA IMAGES

GTX66604 WB Image

WB analysis of HeLa cells treated by 10% FBS at 37ºC for 30 minutes after serum-starvation overnight and NIH/3T3 cells treated by 10% FBS at 37ºC for 30 minutes after serum-starvation overnight using GTX66604 RPS6 (phospho Ser240/Ser244) antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX66604 IP Image

IP analysis of 293T cell lysate using GTX66604 RPS6 (phospho Ser240/Ser244) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty