GeneTex
United States (US)

RPSA antibody [N1C3]

GTX100831_IHC_19011506_485.jpg
GTX100831_40058_20150226_IHC_M_19011506_685.jpg
GTX100831_40639_WB_19011506_898.jpg
GTX100831_40639_WB_2_19011506_455.jpg
GTX100831_40058_WB_M_brain_19011506_872.jpg

Cat No. GTX100831

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HepG2 , Jurkat , Mouse brain

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPSA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

37LRP antibody, 67LR antibody, LAMBR antibody, LAMR1 antibody, LBP antibody, LRP antibody, p40 antibody, RPSA antibody, multidrug resistance-associated protein MGr1-Ag antibody, laminin binding protein antibody, 40S ribosomal protein SA antibody, 37 kDa laminin receptor antibody, 37/67 kDa laminin receptor antibody, 67 kDa laminin receptor antibody, "67kD, ribosomal protein SA antibody", laminin-binding protein precursor p40 antibody, "laminin receptor 1 (67kD, ribosomal protein SA) antibody", colon carcinoma laminin-binding protein antibody, ribosomal protein SA antibody

Cellular Localization

Cell membrane , Cytoplasm (By similarity) , Nucleus (By similarity)

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100831_IHC_19011506_485.jpg

GTX100831 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using RPSA(GTX100831) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100831_40058_20150226_IHC_M_19011506_685.jpg

GTX100831 IHC-P Image

RPSA antibody [N1C3] detects RPSA protein at cytoplasm on mouse small intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse small intestine.
RPSA antibody [N1C3] (GTX100831) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100831_40639_WB_19011506_898.jpg

GTX100831 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
GTX100831 diluted at 1:1000

GTX100831_40639_WB_2_19011506_455.jpg

GTX100831 WB Image

RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody (GTX100831) diluted by 1:1000

GTX100831_40058_WB_M_brain_19011506_872.jpg

GTX100831 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX100831 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169