GeneTex
United States (US)

RRAS antibody [N1C3]

GTX105575_39771_20160303_WB_R_19011506_124.jpg
GTX105575_39771_IHC_19011506_175.jpg
GTX105575_39771_20150206_IHC_M_19011506_765.jpg
GTX105575_39771_20160303_WB_M_19011506_669.jpg
GTX105575_WB_19011506_901.jpg

Cat No. GTX105575

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , Molt-4 , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRAS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RRAS antibody, p23 antibody, Oncogene RRAS antibody, ras-related protein R-Ras antibody, related RAS viral (r-ras) oncogene homolog antibody

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity)

Background

Regulates the organization of the actin cytoskeleton.
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Database

DATA IMAGES
GTX105575_39771_20160303_WB_R_19011506_124.jpg

GTX105575 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RRAS antibody [N1C3] (GTX105575) diluted at 1:500.

GTX105575_39771_IHC_19011506_175.jpg

GTX105575 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hep3B xenograft, using RRAS(GTX105575) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105575_39771_20150206_IHC_M_19011506_765.jpg

GTX105575 IHC-P Image

RRAS antibody [N1C3] detects RRAS protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
RRAS antibody [N1C3] (GTX105575) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105575_39771_20160303_WB_M_19011506_669.jpg

GTX105575 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RRAS antibody [N1C3] (GTX105575) diluted at 1:500.

GTX105575_WB_19011506_901.jpg

GTX105575 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
B:Raji(GTX27908)
12% SDS PAGE
GTX105575 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169