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RRM1 antibody [HL1231]

Recombinant antibody
Anti-RRM1 antibody [HL1231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636579
Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579
Anti-RRM1 antibody [HL1231] used in IHC (Paraffin sections) (IHC-P). GTX636579
Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579
Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579
Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579

Cat. No. GTX636579

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1231

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all RRM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910013

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonucleotide reductase catalytic subunit M1 , R1 , RIR1 , RR1

Cellular Localization

Cytoplasm

Background

This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-RRM1 antibody [HL1231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636579

GTX636579 ICC/IF Image

RRM1 antibody [HL1231] detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RRM1 stained by RRM1 antibody [HL1231] (GTX636579) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579

GTX636579 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody [HL1231] (GTX636579) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RRM1 antibody [HL1231] used in IHC (Paraffin sections) (IHC-P). GTX636579

GTX636579 IHC-P Image

RRM1 antibody [HL1231] detects RRM1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RRM1 stained by RRM1 antibody [HL1231] (GTX636579) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579

GTX636579 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody [HL1231] (GTX636579) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579

GTX636579 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody [HL1231] (GTX636579) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RRM1 antibody [HL1231] used in Western Blot (WB). GTX636579

GTX636579 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody [HL1231] (GTX636579) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations