GeneTex
United States (US)

RRM1 antibody

GTX100758_40618_IHC_2_19011506_105.jpg
GTX100758_40618_IHC_19011506_822.jpg
GTX100758_40618_WB_M_19011506_621.jpg
GTX100758_40618_WB_19011506_322.jpg
GTX100758_40618_IFA_19011506_186.jpg

Cat No. GTX100758

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
See all RRM1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

293T , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribonucleotide Reductase Catalytic Subunit M1,R1,Rir1,Rr1,Rrm1

Cellular Localization

Cytoplasm

Background

This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100758_40618_IHC_2_19011506_105.jpg

GTX100758 IHC-P Image

RRM1 antibody detects RRM1 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
RRM1 antibody (GTX100758) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX100758_40618_IHC_19011506_822.jpg

GTX100758 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RRM1(GTX100758) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX100758_40618_WB_M_19011506_621.jpg

GTX100758 WB Image

RRM1 antibody detects RRM1 protein by Western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg Raw264.7 whole cell lysate/extract
C. 30 µg C2C12 whole cell lysate/extract
5 % SDS-PAGE
RRM1 antibody (GTX100758) dilution: 1:1000
GTX100758_40618_WB_19011506_322.jpg

GTX100758 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
GTX100758 diluted at 1:2000

GTX100758_40618_IFA_19011506_186.jpg

GTX100758 ICC/IF Image

RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: RRM1 protein stained by RRM1 antibody (GTX100758) diluted at 1:1000.
Blue: Hoechst 33342 staining.
REFERENCE

Application Reference

Hsieh YY et al. Cell Death Discovery 2016; 2 : 16027 Repositioning of a cyclin-dependent kinase inhibitor GW8510 as a ribonucleotide reductase M2 inhibitor to treat human colorectal cancer.
Application : WB
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169