APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RRM1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ribonucleotide reductase catalytic subunit M1,R1,RIR1,RR1
Cellular Localization
Cytoplasm
Background
This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100758 IHC-P Image
RRM1 antibody detects RRM1 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
RRM1 antibody (GTX100758) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100758 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RRM1(GTX100758) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100758 WB Image
RRM1 antibody detects RRM1 protein by Western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg Raw264.7 whole cell lysate/extract
C. 30 µg C2C12 whole cell lysate/extract
5 % SDS-PAGE
RRM1 antibody (GTX100758) dilution: 1:1000
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GTX100758 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
GTX100758 diluted at 1:2000
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GTX100758 ICC/IF Image
RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: RRM1 protein stained by RRM1 antibody (GTX100758) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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REFERENCE
REVIEW
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