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RRM2 antibody [HL1235]

Recombinant antibody Orthogonal
Anti-RRM2 antibody [HL1235] used in Western Blot (WB). GTX636634
Anti-RRM2 antibody [HL1235] used in Western Blot (WB). GTX636634
Anti-RRM2 antibody [HL1235] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636634
Anti-RRM2 antibody [HL1235] used in IHC (Paraffin sections) (IHC-P). GTX636634
Anti-RRM2 antibody [HL1235] used in IHC (Whole mount) (IHC-Wm). GTX636634

Cat. No. GTX636634

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1235

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RRM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910018

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonucleotide reductase regulatory subunit M2 , R2 , RR2 , RR2M

Cellular Localization

Cytoplasm

Background

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-RRM2 antibody [HL1235] used in Western Blot (WB). GTX636634

GTX636634 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [HL1235] (GTX636634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RRM2 antibody [HL1235] used in Western Blot (WB). GTX636634

GTX636634 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [HL1235] (GTX636634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RRM2 antibody [HL1235] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636634

GTX636634 ICC/IF Image

RRM2 antibody [HL1235] detects RRM2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RRM2 stained by RRM2 antibody [HL1235] (GTX636634) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RRM2 antibody [HL1235] used in IHC (Paraffin sections) (IHC-P). GTX636634

GTX636634 IHC-P Image

RRM2 antibody [HL1235] detects RRM2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RRM2 stained by RRM2 antibody [HL1235] (GTX636634) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RRM2 antibody [HL1235] used in IHC (Whole mount) (IHC-Wm). GTX636634

GTX636634 IHC-Wm Image

RRM2 antibody [HL1235] detects RRM2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RRM2 stained by RRM2 antibody [HL1235] (GTX636634) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations