GeneTex
United States (US)

RRM2 antibody [N1C1]

GTX103193_42151_20170322_IHC-P_R_19011506_333.jpg
GTX103193_40128_20141108_IHC_M_2_19011506_446.jpg
GTX103193_42718_20161123_IFA_19011506_589.jpg
GTX103193_42718_20170322_IHC-P_M_19011506_572.jpg
GTX103193_41745_20150513_IFA_19011506_264.jpg
GTX103193_42718_20170322_IHC-P_R_19011506_463.jpg

Cat No. GTX103193

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribonucleotide Reductase Regulatory Subunit M2,R2,Rr2,Rr2M,Rrm2

Cellular Localization

Cytoplasm

Background

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103193_42151_20170322_IHC-P_R_19011506_333.jpg

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103193_40128_20141108_IHC_M_2_19011506_446.jpg

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103193_42718_20161123_IFA_19011506_589.jpg

GTX103193 ICC/IF Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RRM2 protein stained by RRM2 antibody [N1C1] (GTX103193) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX103193_42718_20170322_IHC-P_M_19011506_572.jpg

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103193_41745_20150513_IFA_19011506_264.jpg

GTX103193 ICC/IF Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RRM2 protein stained by RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX103193_42718_20170322_IHC-P_R_19011506_463.jpg

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169