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RRM2 antibody

Anti-RRM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00888
Anti-RRM2 antibody used in Immunoprecipitation (IP). GTX00888
Anti-RRM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00888
Anti-RRM2 antibody used in Western Blot (WB). GTX00888

Cat No. GTX00888

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Hamster, Xenopus
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:300-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (11 amino acids) in the C-terminal region of human and mouse RRM2, conjugated with KLH

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribonucleotide reductase regulatory subunit M2 , R2 , RR2 , RR2M , RRM2

Cellular Localization

Cytoplasm

Background

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-RRM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00888

GTX00888 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX00888 RRM2 antibody.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100
Dilution : 1:100

Anti-RRM2 antibody used in Immunoprecipitation (IP). GTX00888

GTX00888 IP Image

IP analysis of CHO whole cell lysate using GTX00888 RRM2 antibody. The upper band in lane 2 is IgG heavy chain.
Lane 1 : Input
Lane 2 : The immunoprecipitate with the antibody
Lane 3 : Supernatant of immuno-precipitation
IP antibody : 1:1000
IB antibody : 1:500

Anti-RRM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00888

GTX00888 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00888 RRM2 antibody. RRM2 is abundantly expressed in actively proliferating cells.
Dilution : 1:300

Anti-RRM2 antibody used in Western Blot (WB). GTX00888

GTX00888 WB Image

WB analysis of various samples using GTX00888 RRM2 antibody. Multiple bands are due to phosphorylation at Ser20 and/or Thr33 (human sequence).
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Lane 4 : Xenopus eggs at mitotic stage
Dilution : 1:1000
Loading : 20 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 359