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RRP1 antibody

Anti-RRP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115107
Anti-RRP1 antibody used in Western Blot (WB). GTX115107
Anti-RRP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115107

Cat. No. GTX115107

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622923

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal RNA processing 1 , D21S2056E , NNP-1 , NOP52 , RRP1A

Cellular Localization

Nucleus , nucleolus

Background

The protein encoded by this gene is the putative homolog of the yeast ribosomal RNA processing protein RRP1. The encoded protein is involved in the late stages of nucleologenesis at the end of mitosis, and may be required for the generation of 28S rRNA. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RRP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115107

GTX115107 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using RRP1(GTX115107) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RRP1 antibody used in Western Blot (WB). GTX115107

GTX115107 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115107 diluted at 1:1000

Anti-RRP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115107

GTX115107 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RRP1(GTX115107) antibody at 1:500 dilution.

REFERENCE

Application Reference

Houston R et al. PLoS Biol 2020; 18 (11) : e3000981 Acetylation-mediated remodeling of the nucleolus regulates cellular acetyl-CoA responses.
Application : WB
Reactivity : Human
Ryan Houston et al. bioRxiv 2020; (Epub) Acetylation-mediated phase control of the nucleolus regulates cellular acetyl-CoA responses.
Application : WB
Reactivity : Human
Yoshikawa H et al. Nucleic Acids Res 2015; 43 (11) : 5524-36 Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169