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RSK2 antibody [4E10]

Anti-RSK2 antibody [4E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60517
Anti-RSK2 antibody [4E10] used in Western Blot (WB). GTX60517
Anti-RSK2 antibody [4E10] used in ELISA (ELISA). GTX60517
Anti-RSK2 antibody [4E10] used in Flow cytometry (FACS). GTX60517
Anti-RSK2 antibody [4E10] used in Western Blot (WB). GTX60517

Cat. No. GTX60517

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all RSK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human RSK2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase A3 , CLS , HU-3 , ISPK-1 , MAPKAPK1B , MRX19 , RSK , RSK2 , S6K-alpha3 , p90-RSK2 , pp90RSK2

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RSK2 antibody [4E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60517

GTX60517 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60517 RSK2 antibody [4E10].
Green : RSK2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-RSK2 antibody [4E10] used in Western Blot (WB). GTX60517

GTX60517 WB Image

WB analysis of human RSK2 (AA: 1-212) recombinant protein using GTX60517 RSK2 antibody [4E10].

Anti-RSK2 antibody [4E10] used in ELISA (ELISA). GTX60517

GTX60517 ELISA Image

ELISA analysis of antigen using GTX60517 RSK2 antibody [4E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-RSK2 antibody [4E10] used in Flow cytometry (FACS). GTX60517

GTX60517 FACS Image

FACS analysis of HepG2 cells using GTX60517 RSK2 antibody [4E10].
Green : RSK2
Red : negative control

Anti-RSK2 antibody [4E10] used in Western Blot (WB). GTX60517

GTX60517 WB Image

WB analysis of HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate using GTX60517 RSK2 antibody [4E10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409