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RSK2 antibody

Comparison
Anti-RSK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105543
Anti-RSK2 antibody used in Western Blot (WB). GTX105543
Anti-RSK2 antibody used in Western Blot (WB). GTX105543
Anti-RSK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105543

Cat. No. GTX105543

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RSK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HepG2 , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RSK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885257

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase A3 , CLS , HU-3 , ISPK-1 , MAPKAPK1B , MRX19 , RSK , RSK2 , S6K-alpha3 , p90-RSK2 , pp90RSK2

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RSK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105543

GTX105543 IHC-P Image

RSK2 antibody detects RSK2 protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RSK2 antibody (GTX105543) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RSK2 antibody used in Western Blot (WB). GTX105543

GTX105543 WB Image

RSK2 antibody detects RSK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK2 antibody (GTX105543) diluted by 1:1000.

Anti-RSK2 antibody used in Western Blot (WB). GTX105543

GTX105543 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with RSK2 antibody (GTX105543) diluted at 1:4000 and competitor's antibody (SC-1430) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RSK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105543

GTX105543 IHC-P Image

RSK2 antibody detects RSK2 protein at cytosol and nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
RSK2 antibody (GTX105543) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169