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RSK3 antibody [N2C1], Internal

Orthogonal Overexpression
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071

Cat. No. GTX111071

Reference 1
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RSK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , DDDDK-tagged RPS6KA2-transfected 293T , Neuro2A , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RSK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951773

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein S6 kinase A2 , HU-2 , MAPKAPK1C , RSK , RSK3 , S6K-alpha , S6K-alpha2 , p90-RSK3 , p90RSK2 , pp90RSK3

Cellular Localization

Nucleus

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071

GTX111071 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071

GTX111071 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071

GTX111071 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tai F et al. Nat Commun 2020; 11 (1) : 258 Enhanced JunD/RSK3 signalling due to loss of BRD4/FOXD3/miR-548d-3p axis determines BET inhibition resistance.
Submit a Reference

REVIEW

RSK3 antibody [N2C1], Internal Cat. No. GTX111071

Rating
( Average 5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 4 users reviews)
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 31937753
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 31937753
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 31937753
Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020. PMID: 31937753
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169