APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , DDDDK-tagged RPS6KA2-transfected 293T , Neuro2A , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RSK3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951773
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S6 kinase A2 , HU-2 , MAPKAPK1C , RSK , RSK3 , S6K-alpha , S6K-alpha2 , p90-RSK3 , p90RSK2 , pp90RSK3
Cellular Localization
Nucleus
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/templates/default/js/lightbox/images/loading.gif "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
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GTX111071 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111071 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111071 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111071 WB Image
The data was published in the journal Nat Commun in 2020. PMID: 31937753
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GTX111071 WB Image
The data was published in the journal Nat Commun in 2020. PMID: 31937753
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GTX111071 WB Image
The data was published in the journal Nat Commun in 2020. PMID: 31937753
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GTX111071 WB Image
The data was published in the journal Nat Commun in 2020. PMID: 31937753
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REFERENCE
REVIEW
There are currently no reviews for RSK3 antibody [N2C1], Internal (GTX111071). Be the first to share your experience with this product.
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_40044_20190118_WB_M_w_23060500_206.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_40044_20181109_WB_w_23060500_920.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_40044_20181221_WB_B_w_23060500_481.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_20240411_citeab_WB_3149_24041502_356.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_20240411_citeab_WB_3150_24041502_862.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_20240411_citeab_WB_3225_24041502_441.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/small/GTX111071/GTX111071_20240411_citeab_WB_2456_24041502_500.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_40044_20190118_WB_M_w_23060500_206.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_40044_20181109_WB_w_23060500_920.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_40044_20181221_WB_B_w_23060500_481.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_20240411_citeab_WB_3149_24041502_356.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_20240411_citeab_WB_3150_24041502_862.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_20240411_citeab_WB_3225_24041502_441.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")
![Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071](/upload/website/prouct_img/normal/GTX111071/GTX111071_20240411_citeab_WB_2456_24041502_500.webp "Anti-RSK3 antibody [N2C1], Internal used in Western Blot (WB). GTX111071")