RSK3 (phospho Thr356/Ser360) antibody [HL3299]

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:1000 and RSK3 antibody [N2C1], Internal (GTX111071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Specificity test was performed by coating the plate with recombinant RPS6KA2 non-phospho peptide, RPS6KA2 (phospho Thr356/Ser360) peptide, RPS6KA2 (phospho Thr356) peptide, and RPS6KA2 (phospho Ser360) peptide (62.5 ng/mL). Coated protein was probed with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

RSK3 (phospho Thr356/Ser360) antibody [HL3299] detects RSK3 (phospho Thr356/Ser360) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RSK3 (phospho Thr356/Ser360) stained by RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.