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RSL1D1 antibody

Anti-RSL1D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48683
Anti-RSL1D1 antibody used in Western Blot (WB). GTX48683

Cat. No. GTX48683

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all RSL1D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
IP 1:100
ELISA 1:2500-1:10000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal sequence of human PBK1.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal L1 domain containing 1 , CSIG , L12 , PBK1 , UTP30

Cellular Localization

Nucleolus

Background

PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004).A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and

Database

Research Area

DATA IMAGES

Anti-RSL1D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48683

GTX48683 ICC/IF Image

Immunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining.

Anti-RSL1D1 antibody used in Western Blot (WB). GTX48683

GTX48683 WB Image

Western blot using GeneTex's affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399