GeneTex
United States (US)

RSPO3 antibody

GTX32155_3278_WB_20180221_18121410_444.jpg
GTX32155_461_ICC-IF_20180221_18121410_736.jpg
GTX32155_460_ICC-IF_20180221_18121410_551.jpg

Cat No. GTX32155

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

Specificity/Sensitivity

At least three isoforms of RSPO3 are known to exist; this antibody will detect all three.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3.The immunogen is located within amino acids 200 - 250 of RSPO3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

R-Spondin 3,Cristin1,Pwtsr,Thsd2,Rspo3

Background

This gene encodes a member of the thrombospondin type 1 repeat supergene family. In addition, the protein contains a furin-like cysteine-rich region. Furin-like repeat domains have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32155_3278_WB_20180221_18121410_444.jpg

GTX32155 WB Image

WB analysis of 293 cell lysate using GTX32155 RSPO3 antibody.
Working concentration : 1 μg/ml

GTX32155_461_ICC-IF_20180221_18121410_736.jpg

GTX32155 ICC/IF Image

ICC/IF analysis of 293 cells using GTX32155 RSPO3 antibody.
Working concentration : 20 μg/ml

GTX32155_460_ICC-IF_20180221_18121410_551.jpg

GTX32155 ICC/IF Image

ICC/IF analysis of 293 cells using GTX32155 RSPO3 antibody.
Working concentration : 5 μg/ml