RTN3 antibody

Cat. No. GTX131091

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all RTN3 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

HeLa membrane fraction extract

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RTN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886420

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

reticulon 3 , ASYIP , HAP , NSPL2 , NSPLII , RTN3-A1

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein

Background

The reticulons are a group of highly conserved genes with preferential expression in neuroendocrine tissues (see, e.g., RTN1; MIM 600865).[supplied by OMIM]

Database

Gene ID: 10313 RTN3
UniProt: O95197 RTN3

Research Area

DATA IMAGES

GTX131091 WB Image

RTN3 antibody detects RTN3 protein by western blot analysis. HeLa whole cell extracts and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RTN3 antibody (GTX131091) diluted at 1:500.

GTX131091 IP Image

Immunoprecipitation of RTN3 protein from HeLa whole cell extracts using 5 μg of RTN3 antibody (GTX131091).
Western blot analysis was performed using RTN3 antibody (GTX131091) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty