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RUFY1 antibody [N2C2], Internal

Anti-RUFY1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119222
Anti-RUFY1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119222
Anti-RUFY1 antibody [N2C2], Internal used in Western Blot (WB). GTX119222

Cat. No. GTX119222

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUFY1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619016

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RUN and FYVE domain containing 1 , RABIP4 , ZFYVE12

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein

Background

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.

Database

Research Area

DATA IMAGES

Anti-RUFY1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119222

GTX119222 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using RUFY1(GTX119222) antibody at 1:200 dilution.

Anti-RUFY1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119222

GTX119222 IHC-P Image

RUFY1 antibody [N2C2], Internal detects RUFY1 protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
RUFY1 antibody [N2C2], Internal (GTX119222) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RUFY1 antibody [N2C2], Internal used in Western Blot (WB). GTX119222

GTX119222 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX119222 diluted at 1:1000

REFERENCE

There are currently no references for RUFY1 antibody [N2C2], Internal (GTX119222). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RUFY1 antibody [N2C2], Internal (GTX119222). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169