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RUFY1 antibody [N3C3]

Anti-RUFY1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119223
Anti-RUFY1 antibody [N3C3] used in Western Blot (WB). GTX119223
Anti-RUFY1 antibody [N3C3] used in Western Blot (WB). GTX119223

Cat. No. GTX119223

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RUFY1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Mouse brown adipose tissue , U87-MG

Predict Reactivity

Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUFY1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632857

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RUN and FYVE domain containing 1 , RABIP4 , ZFYVE12

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein

Background

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.

Database

Research Area

DATA IMAGES

Anti-RUFY1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119223

GTX119223 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using RUFY1(GTX119223) antibody at 1:500 dilution.

Anti-RUFY1 antibody [N3C3] used in Western Blot (WB). GTX119223

GTX119223 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brown adipose tissue
7.5% SDS PAGE
GTX119223 diluted at 1:1000

Anti-RUFY1 antibody [N3C3] used in Western Blot (WB). GTX119223

GTX119223 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX119223 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169