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RUNX1 antibody [5A1]

Anti-RUNX1 antibody [5A1] used in ELISA (ELISA). GTX60402
Anti-RUNX1 antibody [5A1] used in Western Blot (WB). GTX60402
Anti-RUNX1 antibody [5A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60402

Cat. No. GTX60402

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A1

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all RUNX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide of human RUNX1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

runt related transcription factor 1 , AML1 , AML1-EVI-1 , AMLCR1 , CBF2alpha , CBFA2 , EVI-1 , PEBP2aB , PEBP2alpha

Cellular Localization

Nucleus

Background

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RUNX1 antibody [5A1] used in ELISA (ELISA). GTX60402

GTX60402 ELISA Image

ELISA analysis of antigen using GTX60402 RUNX1 antibody [5A1].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-RUNX1 antibody [5A1] used in Western Blot (WB). GTX60402

GTX60402 WB Image

WB analysis of Jurkat cell lysate using GTX60402 RUNX1 antibody [5A1].

Anti-RUNX1 antibody [5A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60402

GTX60402 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60402 RUNX1 antibody [5A1].
Green : RUNX1
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409