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RUNX2 antibody

Anti-RUNX2 antibody used in Western Blot (WB). GTX00792
Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00792
Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00792
Anti-RUNX2 antibody used in Western Blot (WB). GTX00792
Anti-RUNX2 antibody used in Western Blot (WB). GTX00792
Anti-RUNX2 antibody used in Western Blot (WB). GTX00792
Anti-RUNX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00792
Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00792

Cat. No. GTX00792

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RUNX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RUNX2, corresponding to a region within the internal amino acids.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AML3 , CBFA1 , CBFalpha1 , CCD , CCD1 , CLCD , OSF2 , PEA2aA , PEBP2aA , RUNX2 , runt related transcription factor 2 , RUNX family transcription factor 2

Cellular Localization

Nucleus

Background

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-RUNX2 antibody used in Western Blot (WB). GTX00792

GTX00792 WB Image

WB analysis of various cell lysates using GTX00792 RUNX2 antibody.
Lane 1 : HeLa cell lysate, blocked with antigen-specific peptides
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate

Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00792

GTX00792 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX00792 RUNX2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00792

GTX00792 IHC-P Image

IHC-P analysis of human bone marrow tissue using GTX00792 RUNX2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-RUNX2 antibody used in Western Blot (WB). GTX00792

GTX00792 WB Image

WB analysis of various tissue lysates using GTX00792 RUNX2 antibody.
Lane 1 : Mouse brain tissue lysate treated with blocking peptide
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat spleen tissue lysate

Anti-RUNX2 antibody used in Western Blot (WB). GTX00792

GTX00792 WB Image

WB analysis of various tissue lysates using GTX00792 RUNX2 antibody.
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse brain tissue lysate

Anti-RUNX2 antibody used in Western Blot (WB). GTX00792

GTX00792 WB Image

WB analysis of HeLa cell lysate using GTX00792 RUNX2 antibody. The lane on the left was treated with the antigen-specific peptide.

Anti-RUNX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00792

GTX00792 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00792 RUNX2 antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX00792

GTX00792 IHC-P Image

IHC-P analysis of rat brain tissue using GTX00792 RUNX2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

REFERENCE

Application Reference

Kaida N et al. Sci Rep 2024; 14 (1) : 25335 Ridge preservation using octacalcium phosphate collagen to induce new bone containing a vascular network of mainly Type H vessels.
Q Wang et al. J Cell Mol Med 2024; 28 (10) : e18385 Lithium prevents glucocorticoid?induced osteonecrosis of the femoral head by regulating autophagy
Zhu X et al. J Orthop Surg Res 2022; 17 (1) : 423 ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399