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RUNX2 (phospho Ser275) antibody

Anti-RUNX2 (phospho Ser275) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00813
Anti-RUNX2 (phospho Ser275) antibody used in IHC (Paraffin sections) (IHC-P). GTX00813
Anti-RUNX2 (phospho Ser275) antibody used in Western Blot (WB). GTX00813
Anti-RUNX2 (phospho Ser275) antibody used in ELISA (ELISA). GTX00813
Anti-RUNX2 (phospho Ser275) antibody used in IHC (Paraffin sections) (IHC-P). GTX00813
Anti-RUNX2 (phospho Ser275) antibody used in Western Blot (WB). GTX00813

Cat No. GTX00813

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RUNX2 around the phosphorylation site of Ser275.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AML3,CBFA1,CBFalpha1,CCD,CCD1,CLCD,OSF2,PEA2aA,PEBP2aA,RUNX2,runt related transcription factor 2,RUNX family transcription factor 2

Cellular Localization

Nucleus

Background

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-RUNX2 (phospho Ser275) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00813

GTX00813 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells with serum starvation using GTX00813 RUNX2 (phospho Ser275) antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-RUNX2 (phospho Ser275) antibody used in IHC (Paraffin sections) (IHC-P). GTX00813

GTX00813 IHC-P Image

IHC-P analysis of rat brain tissue using GTX00813 RUNX2 (phospho Ser275) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-RUNX2 (phospho Ser275) antibody used in Western Blot (WB). GTX00813

GTX00813 WB Image

WB analysis of mouse thymus tissue using GTX00813 RUNX2 (phospho Ser275) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-RUNX2 (phospho Ser275) antibody used in ELISA (ELISA). GTX00813

GTX00813 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00813 RUNX2 (phospho Ser275) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-RUNX2 (phospho Ser275) antibody used in IHC (Paraffin sections) (IHC-P). GTX00813

GTX00813 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX00813 RUNX2 (phospho Ser275) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-RUNX2 (phospho Ser275) antibody used in Western Blot (WB). GTX00813

GTX00813 WB Image

WB analysis of mouse thymus tissue using GTX00813 RUNX2 (phospho Ser275) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

REFERENCE

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REVIEW

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Package List Price ($)
$ 329