APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Chicken, Pig, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human RUNX2 around the phosphorylation site of S28.
Purification
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AML3 , CBFA1 , CBFalpha1 , CCD , CCD1 , CLCD , OSF2 , PEA2aA , PEBP2aA , RUNX2 , runt related transcription factor 2 , RUNX family transcription factor 2
Cellular Localization
Nucleus
Background
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
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GTX00811 WB Image
WB analysis of heat-shock treated HepG2 cell lysate using GTX00811 RUNX2 (phospho Ser28) antibody. The lane on the left was treated with blocking peptide.
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GTX00811 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00811 RUNX2 (phospho Ser28) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX00811 IHC-P Image
IHC-P analysis of rat lung tissue using GTX00811 RUNX2 (phospho Ser28) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00811 IHC-P Image
IHC-P analysis of rat heart tissue using GTX00811 RUNX2 (phospho Ser28) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00811 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX00811 RUNX2 (phospho Ser28) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00811 WB Image
WB analysis of mouse thymus tissue lysate using GTX00811 RUNX2 (phospho Ser28) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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GTX00811 ICC/IF Image
ICC/IF analysis of PFA-fixed HepG2 cells with LPS treatment for 4hrs using GTX00811 RUNX2 (phospho Ser28) antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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GTX00811 WB Image
WB analysis of mouse thymus tissue lysate using GTX00811 RUNX2 (phospho Ser28) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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REFERENCE
There are currently no references for RUNX2 (phospho Ser28) antibody (GTX00811). Be the first to share your publications with this product.
REVIEW
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