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RUNX2 (phospho Ser340) antibody

Anti-RUNX2 (phospho Ser340) antibody used in ELISA (ELISA). GTX00812
Anti-RUNX2 (phospho Ser340) antibody used in Western Blot (WB). GTX00812
Anti-RUNX2 (phospho Ser340) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00812

Cat No. GTX00812

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RUNX2 around the phosphorylation site of S340.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AML3 , CBFA1 , CBFalpha1 , CCD , CCD1 , CLCD , OSF2 , PEA2aA , PEBP2aA , RUNX2 , runt related transcription factor 2 , RUNX family transcription factor 2

Cellular Localization

Nucleus

Background

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-RUNX2 (phospho Ser340) antibody used in ELISA (ELISA). GTX00812

GTX00812 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00812 RUNX2 (phospho Ser340) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-RUNX2 (phospho Ser340) antibody used in Western Blot (WB). GTX00812

GTX00812 WB Image

WB analysis of mouse thymus tissue lysate using GTX00812 RUNX2 (phospho Ser340) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-RUNX2 (phospho Ser340) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00812

GTX00812 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells with LPS treatment for 4hrs using GTX00812 RUNX2 (phospho Ser340) antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

There are currently no references for RUNX2 (phospho Ser340) antibody (GTX00812). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RUNX2 (phospho Ser340) antibody (GTX00812). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329