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RUNX3 antibody [2B3]

Anti-RUNX3 antibody [2B3] used in ELISA (ELISA). GTX60639
Anti-RUNX3 antibody [2B3] used in IHC (Paraffin sections) (IHC-P). GTX60639
Anti-RUNX3 antibody [2B3] used in Flow cytometry (FACS). GTX60639
Anti-RUNX3 antibody [2B3] used in Western Blot (WB). GTX60639

Cat. No. GTX60639

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B3

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all RUNX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human RUNX3 (AA:186-252) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

runt related transcription factor 3 , AML2 , CBFA3 , PEBP2aC

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RUNX3 antibody [2B3] used in ELISA (ELISA). GTX60639

GTX60639 ELISA Image

ELISA analysis of antigen using GTX60639 RUNX3 antibody [2B3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-RUNX3 antibody [2B3] used in IHC (Paraffin sections) (IHC-P). GTX60639

GTX60639 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60639 RUNX3 antibody [2B3].

Anti-RUNX3 antibody [2B3] used in Flow cytometry (FACS). GTX60639

GTX60639 FACS Image

FACS analysis of NIH3T3 cells using GTX60639 RUNX3 antibody [2B3].
Green : RUNX3
Red : negative control

Anti-RUNX3 antibody [2B3] used in Western Blot (WB). GTX60639

GTX60639 WB Image

WB analysis of HEK293 (1) and RUNX3 (AA: 186-252)-hIgGFc transfected HEK293 (2) cell lysate using GTX60639 RUNX3 antibody [2B3].

REFERENCE

Application Reference

Deng R et al. Mol Cancer 2020; 19 (1) : 122 Disruption of super-enhancer-driven tumor suppressor gene RCAN1.4 expression promotes the malignancy of breast carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409