APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A20 Cell Lysate(GTX27180)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
RUSC1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RUSC1.The immunogen is located within amino acids 780 - 830 of RUSC1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RUN and SH3 domain containing 1 , NESCA
Cellular Localization
Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cytoplasmic vesicle,Golgi apparatus
Background
RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IkappaB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-kappaB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-kappaB activation pathway.
Database
Research Area
DATA IMAGES
|
GTX85244 WB Image
WB analysis of A-20 cell lysate using GTX85244 RUSC1 antibody.
Working concentration : 1 μg/ml
|
REFERENCE
There are currently no references for RUSC1 antibody (GTX85244). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RUSC1 antibody (GTX85244). Be the first to share your experience with this product.
