APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT116 , MCF-7 , 293T
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.64 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11165529
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RuvB like AAA ATPase 1 , ECP-54 , ECP54 , INO80H , NMP 238 , NMP238 , PONTIN , Pontin52 , RVB1 , TIH1 , TIP49 , TIP49A
Cellular Localization
Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane
Background
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.
Database
Research Area
DATA IMAGES
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GTX109494 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX109494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109494 WB Image
Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
10% SDS PAGE
GTX109494 diluted at 1:1000
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GTX109494 IP Image
Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 μg of RUVBL1 antibody (GTX109494).
Western blot analysis was performed using RUVBL1 antibody (GTX109494).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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REFERENCE
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REVIEW
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