*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , H1299 , HCT116 , MCF-7
Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris-Glycine (pH7), 20% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.64 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
RuvB like AAA ATPase 1 , ECP-54 , ECP54 , INO80H , NMP 238 , NMP238 , PONTIN , Pontin52 , RVB1 , TIH1 , TIP49 , TIP49A
Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.
GTX109494 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX109494) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109494 WB Image
Sample (30 ug of whole cell lysate)
10% SDS PAGE
GTX109494 diluted at 1:1000
GTX109494 IP Image
Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 μg of RUVBL1 antibody (GTX109494).
Western blot analysis was performed using RUVBL1 antibody (GTX109494).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.