APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11167321
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RuvB like AAA ATPase 1 , ECP-54 , ECP54 , INO80H , NMP 238 , NMP238 , PONTIN , Pontin52 , RVB1 , TIH1 , TIP49 , TIP49A
Cellular Localization
Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane
Background
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.
Database
Research Area
DATA IMAGES
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GTX111294 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111294 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111294 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.
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GTX111294 IHC-P Image
RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111294 IHC-P Image
RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111294 IP Image
Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 μg of RUVBL1 antibody (GTX111294).
Western blot analysis was performed using RUVBL1 antibody (GTX111294).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX111294 ICC/IF Image
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX111294 IHC-P Image
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111294 IHC-P Image
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111294 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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