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RUVBL1 antibody

Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294
Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294
Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294
Anti-RUVBL1 antibody used in Immunoprecipitation (IP). GTX111294
Anti-RUVBL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111294
Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294
Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294
Anti-RUVBL1 antibody used in Western Blot (WB). GTX111294
Anti-RUVBL1 antibody used in Western Blot (WB). GTX111294

Cat No. GTX111294

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167321

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RuvB like AAA ATPase 1 , ECP-54 , ECP54 , INO80H , NMP 238 , NMP238 , PONTIN , Pontin52 , RVB1 , TIH1 , TIP49 , TIP49A

Cellular Localization

Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane

Background

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.

Database

Research Area

DATA IMAGES

Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294

GTX111294 IHC-P Image

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294

GTX111294 IHC-P Image

RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294

GTX111294 IHC-P Image

RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RUVBL1 antibody used in Immunoprecipitation (IP). GTX111294

GTX111294 IP Image

Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 μg of RUVBL1 antibody (GTX111294).
Western blot analysis was performed using RUVBL1 antibody (GTX111294).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RUVBL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111294

GTX111294 ICC/IF Image

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294

GTX111294 IHC-P Image

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RUVBL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111294

GTX111294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RUVBL1 antibody used in Western Blot (WB). GTX111294

GTX111294 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RUVBL1 antibody used in Western Blot (WB). GTX111294

GTX111294 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf