APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RUVBL2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951795
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
RuvB like AAA ATPase 2 , CGI-46 , ECP-51 , ECP51 , INO80J , REPTIN , RVB2 , TAP54-beta , TIH2 , TIP48 , TIP49B
Cellular Localization
Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane
Background
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-RUVBL2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102037](/upload/website/prouct_img/middle/GTX102037/GTX102037_40114_IHC_19011506_550.jpg "Anti-RUVBL2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102037")
|
GTX102037 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human testis, using RUVBL2(GTX102037) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
![Anti-RUVBL2 antibody [C1C3] used in Western Blot (WB). GTX102037](/upload/website/prouct_img/middle/GTX102037/GTX102037_WB_19011506_508.jpg "Anti-RUVBL2 antibody [C1C3] used in Western Blot (WB). GTX102037")
|
GTX102037 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX102037 diluted at 1:5000
|
REFERENCE
There are currently no references for RUVBL2 antibody [C1C3] (GTX102037). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RUVBL2 antibody [C1C3] (GTX102037). Be the first to share your experience with this product.
![Anti-RUVBL2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102037](/upload/website/prouct_img/small/GTX102037/GTX102037_40114_IHC_19011506_550.jpg "Anti-RUVBL2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102037")
![Anti-RUVBL2 antibody [C1C3] used in Western Blot (WB). GTX102037](/upload/website/prouct_img/small/GTX102037/GTX102037_WB_19011506_508.jpg "Anti-RUVBL2 antibody [C1C3] used in Western Blot (WB). GTX102037")
![Anti-RUVBL2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102037](/upload/website/prouct_img/normal/GTX102037/GTX102037_40114_IHC_19011506_550.jpg "Anti-RUVBL2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102037")
![Anti-RUVBL2 antibody [C1C3] used in Western Blot (WB). GTX102037](/upload/website/prouct_img/normal/GTX102037/GTX102037_WB_19011506_508.jpg "Anti-RUVBL2 antibody [C1C3] used in Western Blot (WB). GTX102037")
![Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765](/upload/website/prouct_img/small/GTX629765/GTX629765_41435_MeDIP_18121411_324.jpg "Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765")
![Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420](/upload/website/prouct_img/small/GTX627420/GTX627420_40878_CT_WB_Knockdown_18121411_718.jpg "Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420")
![Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011](/upload/website/prouct_img/small/GTX116011/GTX116011_42732_20180316_WB_KO_watermark_18121410_274.jpg "Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011")
