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RUVBL2 antibody [N1C1]

Anti-RUVBL2 antibody [N1C1] used in Immunoprecipitation (IP). GTX113926
Anti-RUVBL2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113926
Anti-RUVBL2 antibody [N1C1] used in Western Blot (WB). GTX113926
Anti-RUVBL2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113926

Cat No. GTX113926

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUVBL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037926

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RuvB like AAA ATPase 2 , CGI-46 , ECP-51 , ECP51 , INO80J , REPTIN , RVB2 , TAP54-beta , TIH2 , TIP48 , TIP49B

Cellular Localization

Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane

Background

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RUVBL2 antibody [N1C1] used in Immunoprecipitation (IP). GTX113926

GTX113926 IP Image

Immunoprecipitation of RUVBL2 protein from 293T whole cell extracts using 5 μg of RUVBL2 antibody [N1C1] (GTX113926).
Western blot analysis was performed using RUVBL2 antibody [N1C1] (GTX113926).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RUVBL2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113926

GTX113926 IHC-P Image

RUVBL2 antibody [N1C1] detects RUVBL2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL2 antibody [N1C1] (GTX113926) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RUVBL2 antibody [N1C1] used in Western Blot (WB). GTX113926

GTX113926 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113926 diluted at 1:1000

Anti-RUVBL2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113926

GTX113926 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using RUVBL2(GTX113926) antibody at 1:200 dilution.

REFERENCE

There are currently no references for RUVBL2 antibody [N1C1] (GTX113926). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RUVBL2 antibody [N1C1] (GTX113926). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf