*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.89 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human RUVBL2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
RuvB like AAA ATPase 2 , CGI-46 , ECP-51 , ECP51 , INO80J , REPTIN , RVB2 , TAP54-beta , TIH2 , TIP48 , TIP49B
Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]
GTX113926 IP Image
Immunoprecipitation of RUVBL2 protein from 293T whole cell extracts using 5 μg of RUVBL2 antibody [N1C1] (GTX113926).
Western blot analysis was performed using RUVBL2 antibody [N1C1] (GTX113926).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX113926 IHC-P Image
RUVBL2 antibody [N1C1] detects RUVBL2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL2 antibody [N1C1] (GTX113926) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX113926 WB Image
Sample (30 ug of whole cell lysate)
10% SDS PAGE
GTX113926 diluted at 1:1000
GTX113926 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RUVBL2(GTX113926) antibody at 1:200 dilution.