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RUVBL2 antibody

Anti-RUVBL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128970
Anti-RUVBL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128970
Anti-RUVBL2 antibody used in Western Blot (WB). GTX128970

Cat No. GTX128970

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUVBL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RuvB like AAA ATPase 2 , CGI-46 , ECP-51 , ECP51 , INO80J , REPTIN , RVB2 , TAP54-beta , TIH2 , TIP48 , TIP49B

Cellular Localization

Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane

Background

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RUVBL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128970

GTX128970 ICC/IF Image

RUVBL2 antibody detects RUVBL2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL2 protein stained by RUVBL2 antibody (GTX128970) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-RUVBL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128970

GTX128970 IHC-P Image

RUVBL2 antibody detects RUVBL2 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL2 antibody (GTX128970) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RUVBL2 antibody used in Western Blot (WB). GTX128970

GTX128970 WB Image

RUVBL2 antibody detects RUVBL2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
RUVBL2 antibody (GTX128970) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169