*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human RUVBL2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
RuvB like AAA ATPase 2 , CGI-46 , ECP-51 , ECP51 , INO80J , REPTIN , RVB2 , TAP54-beta , TIH2 , TIP48 , TIP49B
Nucleus matrix , Nucleus , nucleoplasm , Cytoplasm , Membrane
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]
GTX128970 ICC/IF Image
RUVBL2 antibody detects RUVBL2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL2 protein stained by RUVBL2 antibody (GTX128970) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX128970 IHC-P Image
RUVBL2 antibody detects RUVBL2 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL2 antibody (GTX128970) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX128970 WB Image
RUVBL2 antibody detects RUVBL2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
RUVBL2 antibody (GTX128970) dilution: 1:1000