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RWDD3 antibody

Anti-RWDD3 antibody used in Western Blot (WB). GTX85246

Cat. No. GTX85246

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RWDD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Rat Kidney Tissue Lysate(GTX27944)

Product Note

This antibody is specific for RWDD3 C-Terminus

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RWDD3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3.The immunogen is located within the last 50 amino acids of RWDD3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RWD domain containing 3 , RSUME

Cellular Localization

Nucleus,Cytoplasm

Background

RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune–inflammatory signals. RWDD3 increases Ikbeta sumoylation and stability. In addition, RWDD3 inhibits TNF-alpha-induced kB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikbeta increased stability. RSUME-enhanced sumoylation of Ikbeta leads to the inhibition of NF-kbeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

Database

Research Area

DATA IMAGES

Anti-RWDD3 antibody used in Western Blot (WB). GTX85246

GTX85246 WB Image

WB analysis of rat kidney tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85246 RWDD3 antibody.
Working concentration : 1 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399