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RYK antibody [F35 P7 D7 F5]

Anti-RYK antibody [F35 P7 D7 F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79478

Cat. No. GTX79478

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F35 P7 D7 F5

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RYK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 2 μg/ml
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C(462) L T E F H A A L G A Y V(475) of RYK, conjugated to ovalbumin.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11169806

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor like tyrosine kinase , D3S3195 , JTK5 , JTK5A , RYK1

Cellular Localization

Membrane,Nucleus,Cytoplasm

Background

The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-RYK antibody [F35 P7 D7 F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79478

GTX79478 ICC/IF Image

ICC/IF analysis of PC-3 cells using GTX79478 RYK antibody [F35 P7 D7 F5]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399