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RYK antibody

Anti-RYK antibody used in IHC (Paraffin sections) (IHC-P). GTX25518
Anti-RYK antibody used in Western Blot (WB). GTX25518

Cat. No. GTX25518

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
400 μl ($399)
See all RYK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat cell lysate

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 561-591 amino acids from the C-terminal region of human RYK.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor like tyrosine kinase , D3S3195 , JTK5 , JTK5A , RYK1

Cellular Localization

Membrane,Nucleus,Cytoplasm

Background

The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-RYK antibody used in IHC (Paraffin sections) (IHC-P). GTX25518

GTX25518 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX25518 RYK antibody.

Anti-RYK antibody used in Western Blot (WB). GTX25518

GTX25518 WB Image

WB analysis of 293 cell lysate using GTX25518 RYK antibody.
Lane 1 : pre-incubated without blocking peptide
Lane 2 : pre-incubate with blocking peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399