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Rab 1A antibody [N1C3]

Orthogonal
Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454
Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454
Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454
Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454

Cat. No. GTX101454

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rab 1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

RAB1A transfected 293T , mouse colon , U87-MG , SK-N-SH , rat brain

Product Note

Based on sequence homology, this antibody may cross react with RAB1B protein.

Predict Reactivity

Sheep, Drosophila, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rab 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951567

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB1A, member RAS oncogene family , RAB1 , YPT1

Cellular Localization

Golgi apparatus , Endoplasmic reticulum

Background

This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454

GTX101454 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454

GTX101454 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454

GTX101454 WB Image

Western Blot analysis of RAB1A expression in transfected 293T cell line by RAB1A polyclonal antibody.
A: Non-transfected lysate.
B: RAB1A transfected lysate.
12% SDS PAGE
GTX101454 diluted at 1:500

Anti-Rab 1A antibody [N1C3] used in Western Blot (WB). GTX101454

GTX101454 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Chao TY et al. Microbiol Spectr 2023; 11 (1) : e0436922 Subcellular Distribution of BALF2 and the Role of Rab1 in the Formation of Epstein-Barr Virus Cytoplasmic Assembly Compartment and Virion Release.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169