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Rab10 antibody [4E2]

Anti-Rab10 antibody [4E2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82800
Anti-Rab10 antibody [4E2] used in Western Blot (WB). GTX82800

Cat. No. GTX82800

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E2

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Rab10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Rab10 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11170606

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB10, member RAS oncogene family

Cellular Localization

Golgi apparatus membrane,Endosome membrane,Endoplasmic reticulum membrane

Background

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Rab10 antibody [4E2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82800

GTX82800 ICC/IF Image

ICC/IF analysis of LOVO cells using GTX82800 Rab10 antibody [4E2].
Green : Rab10
Blue: DRAQ5 fluorescent DNA dye

Anti-Rab10 antibody [4E2] used in Western Blot (WB). GTX82800

GTX82800 WB Image

WB analysis of HeLa (1) and NIH3T3 (2) cell lysate using GTX82800 Rab10 antibody [4E2].

REFERENCE

Application Reference

Peng X et al. Biochim Biophys Acta Mol Cell Res 2021; 1868 (1) : 118857 IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.
Application : WB
Reactivity : Human
Zhipeng Li et al. Hepatology 2020; 72 (2) : 486-502 Maturation of Lipophagic Organelles in Hepatocytes Is Dependent Upon a Rab10/Dynamin-2 Complex.
Reactivity : Human
Ryan J Schulze et al. Proc Natl Acad Sci U S A 2020; 117 (51) : 32443-32452 Direct lysosome-based autophagy of lipid droplets in hepatocytes.
Application : ICC/IF
Reactivity : Mouse
Li Z et al. Sci Adv 2016; 2 (12) : e1601470 A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399