Rab11A antibody [4H9]

Cat. No. GTX03669

Host	Mouse
Clonality	Monoclonal
Clone Name	4H9
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat
Package	100 μg ($449)

See all Rab11A products

Datasheet

View product citations for antibody GTX03669 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.25-0.5 μg/ml
ICC/IF	5 μg/ml
IHC-P	2-5 μg/ml
FACS	1-3 μg/1x10⁶cells

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Rab11A (171-211aa EIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQ), identical to the related mouse and rat sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB11A, member RAS oncogene family , YL8

Cellular Localization

Cell membrane

Background

The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Database

Gene ID: 8766 RAB11A
UniProt: P62491 RAB11A

Research Area

DATA IMAGES

GTX03669 IHC-P Image

IHC-P analysis of human placenta tissue using GTX03669 Rab11A antibody [4H9].
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution)
Dilution : 2 μg/ml

GTX03669 FACS Image

FACS analysis of ANA-1 cells using GTX03669 Rab11A antibody [4H9].
Antibody amount : 1 μg / 1x10⁶ cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

GTX03669 IHC-P Image

IHC-P analysis of human spleen tissue using GTX03669 Rab11A antibody [4H9].
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution)
Dilution : 2 μg/ml

GTX03669 IHC-P Image

IHC-P analysis of human gastric carcinoma tissue using GTX03669 Rab11A antibody [4H9].
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution)
Dilution : 2 μg/ml

GTX03669 WB Image

WB analysis of various sample lysates using GTX03669 Rab11A antibody [4H9].
Lane 1 : HeLa whole cell lysates
Lane 2 : human placenta tissue lysates
Lane 3 : human Colo320 lysates
Lane 4 : human MDA-MB453 whole cell lysates
Lane 5 : rat testis tissue lysates
Lane 6 : rat brain tissue lysates
Lane 7 : mouse testis tissue lysates
Lane 8 : mouse brain tissue lysates
Dilution : 0.5 μg/ml
Loading : 30μg per lane

GTX03669 FACS Image

FACS analysis of U937 cells using GTX03669 Rab11A antibody [4H9].
Antibody amount : 1 μg / 1x10⁶ cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

GTX03669 FACS Image

FACS analysis of RH35 cells using GTX03669 Rab11A antibody [4H9].
Antibody amount : 1 μg / 1x10⁶ cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

GTX03669 ICC/IF Image

ICC/IF analysis of T-47D cells using GTX03669 Rab11A antibody [4H9]. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 5 μg/mL

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Rab11A antibody [4H9]

Cat. No. GTX03669

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX03669 IHC-P Image

GTX03669 FACS Image

GTX03669 IHC-P Image

GTX03669 IHC-P Image

GTX03669 WB Image

GTX03669 FACS Image

GTX03669 FACS Image

GTX03669 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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