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Rab11a + Rab11b antibody

Anti-Rab11a + Rab11b antibody used in Western Blot (WB). GTX127328
Anti-Rab11a + Rab11b antibody used in Immunohistochemistry (IHC). GTX127328
Anti-Rab11a + Rab11b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127328

Cat. No. GTX127328

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Rab11a + Rab11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-Wm Assay dependent
IHC 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Drosophila (93%)| Bovine (100%)| Chicken (99%)| Pig (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Rab11a + Rab11b

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171170

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB11B, member RAS oncogene family, a , RAB11B, member RAS oncogene family, b , RAB11a, member RAS oncogene family , rab11b , wu:fb07g11 , zgc:55760 , zgc:92772 , wu:fi15h09 , zgc:103679

Database

Research Area

DATA IMAGES

Anti-Rab11a + Rab11b antibody used in Western Blot (WB). GTX127328

GTX127328 WB Image

Zebrafish tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rab11a + Rab11b antibody (GTX127328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rab11a + Rab11b antibody used in Immunohistochemistry (IHC). GTX127328

GTX127328 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using Rab11a + Rab11b antibody (GTX127328) at 1:200 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-Rab11a + Rab11b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127328

GTX127328 ICC/IF Image

The data was published in the journal PLoS Genet in 2014.PMID: 24967841

REFERENCE

Application Reference

Karolczak S et al. J Clin Invest 2023; 133 (18) Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy.
Eno C et al. Development 2016; 143 (9) : 1585-99 aura (mid1ip1l) regulates the cytoskeleton at the zebrafish egg-to-embryo transition.
Application : IHC-Wm
Reactivity : Zebrafish
Kanagaraj P et al. PLoS Genet 2014; 10 (6) : e1004449 Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis.
Application : ICC/IF
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169