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Rab25 antibody [3F12]

Anti-Rab25 antibody [3F12] used in Western Blot (WB). GTX82785
Anti-Rab25 antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82785
Anti-Rab25 antibody [3F12] used in IHC (Paraffin sections) (IHC-P). GTX82785
Anti-Rab25 antibody [3F12] used in Flow cytometry (FACS). GTX82785

Cat. No. GTX82785

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Rab25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of Rab25 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11171870

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAB25, member RAS oncogene family , CATX-8 , RAB11C

Cellular Localization

Cell membrane,Cytoplasmic vesicle

Background

RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Rab25 antibody [3F12] used in Western Blot (WB). GTX82785

GTX82785 WB Image

WB analysis of MCF-7 (1), T47D (2) and GC7901 (3) cell lysate using GTX82785 Rab25 antibody [3F12].

Anti-Rab25 antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82785

GTX82785 ICC/IF Image

ICC/IF analysis of A549 cells using GTX82785 Rab25 antibody [3F12].
Green : Rab25
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Rab25 antibody [3F12] used in IHC (Paraffin sections) (IHC-P). GTX82785

GTX82785 IHC-P Image

IHC-P analysis of esophagus tissue (left) and human lung cancer (right) using GTX82785 Rab25 antibody [3F12].

Anti-Rab25 antibody [3F12] used in Flow cytometry (FACS). GTX82785

GTX82785 FACS Image

FACS analysis of NIH3T3 cells using GTX82785 Rab25 antibody [3F12].
Green : Rab25
Purple : negative control

REFERENCE

Application Reference

Qiu S et al. Biomed Pharmacother 2019; 109 : 1841-1850 The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399