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Rab5 antibody

Anti-Rab5 antibody used in Western Blot (WB). GTX13253

Cat No. GTX13253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr, IP, IHC
Reactivity Human, Mouse, Rat, Zebrafish, Bovine, Dog, Hamster, Guinea pig, Pig, Monkey
Package
100 μl ($369)
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.8 kDa). An additional higher molecular mass band of unknown identity at ~45 kDa is detected in some samples on immunoblots. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Mouse or Rat Brain Tissue Extract
PROPERTIES

Form

Liquid

Buffer

Preservative: None Constituents: Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

whole antiserum

Antigen Species

Human

Immunogen

Synthetic peptide: PKNEPQNPGANSARGR conjugated to KLH, corresponding to amino acids 182-197 of Human Rab5.

Purification

Whole antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P20339, Rab5, 5868, 179512, RAB5A

Cellular Localization

At the cytoplasmic surface of the plasma membrane and on early endosomes.

Background

Rab5 can be used as a marker for early endosomes. See Clague et al. 1998 for more information.

Research Area

DATA IMAGES
Anti-Rab5 antibody used in Western Blot (WB). GTX13253

GTX13253 WB Image

Western blot analysis of human HeLa (heat shocked) cell lysate, probed with Rab5 pAb.

REFERENCE
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Package List Price ($)
$ 369