GeneTex
United States (US)

Rab5b antibody

GTX106661_39974_20170908_WB_Fraction_19011506_917.jpg

Cat No. GTX106661

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HeLa , HeLa membrane extract

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rab5b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rab5B, Member Ras Oncogene Family,Rab5B

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side , Early endosome membrane; Lipid-anchor , Melanosome

Background

Protein transport. Probably involved in vesicular traffic.

Database

DATA IMAGES
GTX106661_39974_20170908_WB_Fraction_19011506_917.jpg

GTX106661 WB Image

HeLa whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rab 5B antibody [N1C3] (GTX106661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169