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Rab9 antibody [Mab9]

Anti-Rab9 antibody [Mab9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22810
Anti-Rab9 antibody [Mab9] used in IHC (Paraffin sections) (IHC-P). GTX22810
Anti-Rab9 antibody [Mab9] used in IHC (Paraffin sections) (IHC-P). GTX22810
Anti-Rab9 antibody [Mab9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22810
Anti-Rab9 antibody [Mab9] used in IHC (Paraffin sections) (IHC-P). GTX22810
Anti-Rab9 antibody [Mab9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22810
Anti-Rab9 antibody [Mab9] used in Western Blot (WB). GTX22810

Cat. No. GTX22810

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Mab9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, IHC, IM

Reactivity

 Human, Rat, Bovine, Dog, Hamster, Monkey, Primate
Package
100 μl ($399)
See all Rab9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:500
IHC-P 1:20
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
IM Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody recognizes prenylated and non-prenylated rab 9. It does not cross-react with other rab family members.

PROPERTIES

Form

Liquid

Buffer

PBS with 1mg/mL BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dog

Immunogen

Recombinant canine rab 9a.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384866

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAB9A, member RAS oncogene family , RAB9

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane

Database

Research Area

DATA IMAGES

Anti-Rab9 antibody [Mab9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22810

GTX22810 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22810 Rab9 antibody [Mab9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Rab9 antibody [Mab9] used in IHC (Paraffin sections) (IHC-P). GTX22810

GTX22810 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX22810 Rab9 antibody [Mab9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Rab9 antibody [Mab9] used in IHC (Paraffin sections) (IHC-P). GTX22810

GTX22810 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22810 Rab9 antibody [Mab9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Rab9 antibody [Mab9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22810

GTX22810 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22810 Rab9 antibody [Mab9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Rab9 antibody [Mab9] used in IHC (Paraffin sections) (IHC-P). GTX22810

GTX22810 IHC-P Image

IHC-P analysis of human stomach tissue using GTX22810 Rab9 antibody [Mab9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Rab9 antibody [Mab9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22810

GTX22810 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22810 Rab9 antibody [Mab9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Rab9 antibody [Mab9] used in Western Blot (WB). GTX22810

GTX22810 WB Image

WB analysis of 25 ug of K562 (lane 1), 293 (lane 2) and Hela (lane 3) using GTX22810 Rab9 antibody [Mab9].
Dilution : 1:2000

REFERENCE

Application Reference

Diaz-Salinas MA et al. J Virol 2014; 88 (8) : 4389-402 Rotaviruses reach late endosomes and require the cation-dependent mannose-6-phosphate receptor and the activity of cathepsin proteases to enter the cell.
Application : ICC/IF
Reactivity : Monkey
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399