Rabbit Anti-Human IgG (Fc) antibody [RM116]

Cat. No. GTX60861

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM116
Isotype	IgG
Application	ICC/IF, IHC-P, FACS, ELISA, IHC, Sandwich ELISA
Reactivity	Human, Monkey
Package	100 μg ($379)

See all Human IgG products

Datasheet

View product citations for antibody GTX60861 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	0.5μg/mL - 2μg/mL
IHC-P	0.5μg/mL - 2μg/mL
FACS	Assay dependent
ELISA	50ng/well - 200ng/well (for Capture) ; 0.05μg/mL - 0.2μg/mL (for Detection)
IHC	0.5μg/mL - 2μg/mL
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX60861, Detection : GTX60920

Not tested in other applications.

Product Note

This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human IgG

Target Isotype

IgG Fc

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Gene ID: 3500 IGHG1
Gene ID: 3501
Gene ID: 3502
Gene ID: 3503
Gene ID: 3492

DATA IMAGES

GTX60861 ELISA Image

Sandwich ELISA analysis of different amount of human IgG using serial diluted GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody and GTX60861 Human IgG (Fc) antibody [RM116] as the capture antibody.

GTX60861 ELISA Image

ELISA analysis of human immunoglobulins (50 ng/well) using GTX60861 Human IgG (Fc) antibody [RM116]. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG.
Dilution : 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody.

GTX60861 ELISA Image

Sandwich ELISA analysis of human plasma using GTX60861 Human IgG (Fc) antibody [RM116] as the capture antibody (100ng/well) and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin)(1 ug/mL) as the detection antibody.

GTX60861 ELISA Image

Sandwich ELISA analysis of human IgG using GTX60861 Human IgG (Fc) antibody [RM116] as the capture antibody (100ng/well) and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin)(1 ug/mL) as the detection antibody.

GTX60861 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60861 Human IgG (Fc) antibody [RM116].

GTX60861 IHC Image

IHC analysis of human tonsil tissue using GTX60861 Human IgG (Fc) antibody [RM116].

GTX60861 ELISA Image

ELISA analysis of 0.1ug/well (2ug/mL, 50uL/well) Rhesus monkey IgG using serial diluted GTX60861 Human IgG (Fc) antibody [RM116].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 379

Qty

Rabbit Anti-Human IgG (Fc) antibody [RM116]

Cat. No. GTX60861

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Target Isotype

Purification

Conjugation

Note

TARGET

Database

DATA IMAGES

GTX60861 ELISA Image

GTX60861 ELISA Image

GTX60861 ELISA Image

GTX60861 ELISA Image

GTX60861 ELISA Image

GTX60861 IHC Image

GTX60861 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.