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Rabbit Anti-Human IgG1 antibody [RM117]

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862
Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862
Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862
Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862
Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862
Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

Cat. No. GTX60862

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM117

Isotype

 IgG

Application

 ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μg ($389)
See all Human IgG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 0.5μg/mL - 2μg/mL
IHC-P 0.5μg/mL - 2μg/mL
FACS Assay dependent
ELISA 50ng/well - 200ng/well (for Capture) ; 0.05μg/mL - 0.2μg/mL (for Detection)
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX60862, Detection : GTX60920
Not tested in other applications.

Product Note

This antibody reacts to the heavy chain of human IgG1. RM117 does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to the hinge region of Human IgG1

Target Isotype

IgG1

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

  • Gene ID: 3500 IGHG1

DATA IMAGES

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

GTX60862 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60862 Human IgG1 antibody [RM117].

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

GTX60862 ELISA Image

ELISA analysis of monkey IgG using GTX60862 Human IgG1 antibody [RM117] and positive control Anti-monkey IgG antibody. The result shows that the GTX60862 does not react to monkey IgG.

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

GTX60862 ELISA Image

ELISA analysis of human IgG1 using GTX60862 Human IgG1 antibody [RM117]. No cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

GTX60862 ELISA Image

Sandwich ELISA analysis of human IgG1 in monkey serum using GTX60862 Human IgG1 antibody [RM117] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

GTX60862 IHC-P Image

IHC-P analysis of tonsil tissue using GTX60862 Human IgG1 antibody [RM117].
Dilution : 1:15,000

Rabbit Anti-Human IgG1 antibody [RM117]. GTX60862

GTX60862 ELISA Image

Sandwich ELISA analysis of human plasma using GTX60862 Human IgG1 antibody [RM117] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 389