Rabbit Anti-Human IgG4 antibody [RM120]

Cat. No. GTX60865

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM120
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μg ($379)

See all Human IgG4 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	1 μg/mL - 10μg/mL
IHC-P	1 μg/mL - 10μg/mL
FACS	Assay dependent
ELISA	50ng/well - 200ng/well (for Capture) ; 0.05μg/mL - 0.2μg/mL (for Detection)
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX60865, Detection : GTX60920

Not tested in other applications.

Product Note

This antibody reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to the hinge region of Human IgG4

Target Isotype

IgG4

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Gene ID: 3503 IGHG4

DATA IMAGES

GTX60865 WB Image

WB analysis of human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms using GTX60865 Human IgG4 antibody [RM120]. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

GTX60865 IHC-P Image

IHC-P analysis of tonsil tissue using GTX60865 Human IgG4 antibody [RM120].
Dilution : 1:50,000

GTX60865 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60865 Human IgG4 antibody [RM120].

GTX60865 ELISA Image

Sandwich ELISA analysis of human plasma using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

GTX60865 ELISA Image

Sandwich ELISA analysis of human IgG4 in monkey serum using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

GTX60865 ELISA Image

ELISA analysis of monkey IgG using GTX60865 Human IgG4 antibody [RM120] and positive control Anti-monkey IgG antibody. The result shows that the GTX60865 does not react to monkey IgG.

GTX60865 IHC-P Image

IHC-P analysis of human lymphoid tissue using GTX60865 Human IgG4 antibody [RM120].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 379

Qty

Rabbit Anti-Human IgG4 antibody [RM120]

Cat. No. GTX60865

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Target Isotype

Purification

Conjugation

Note

TARGET

Database

DATA IMAGES

GTX60865 WB Image

GTX60865 IHC-P Image

GTX60865 ELISA Image

GTX60865 ELISA Image

GTX60865 ELISA Image

GTX60865 ELISA Image

GTX60865 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.