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Rabbit Anti-Human IgG4 antibody [RM120]

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865
Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865
Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865
Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865
Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865
Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865
Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

Cat No. GTX60865

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM120

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL - 2 μg/mL
ICC/IF 1 μg/mL - 10μg/mL
IHC-P 1 μg/mL - 10μg/mL
FACS Assay dependent
ELISA 50ng/well - 200ng/well (for Capture) ; 0.05μg/mL - 0.2μg/mL (for Detection)
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX60865, Detection : GTX60920

Not tested in other applications.

Product Note

This antibody reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to the hinge region of Human IgG4

Target Isotype

IgG4

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Database

  • Gene ID: 3503 IGHG4

Research Area

DATA IMAGES

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 ELISA Image

Sandwich ELISA analysis of human plasma using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 ELISA Image

Sandwich ELISA analysis of human IgG4 in monkey serum using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 WB Image

WB analysis of human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms using GTX60865 Human IgG4 antibody [RM120]. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 IHC-P Image

IHC-P analysis of human lymphoid tissue using GTX60865 Human IgG4 antibody [RM120].

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 IHC-P Image

IHC-P analysis of tonsil tissue using GTX60865 Human IgG4 antibody [RM120].
Dilution : 1:50,000

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 ELISA Image

ELISA analysis of monkey IgG using GTX60865 Human IgG4 antibody [RM120] and positive control Anti-monkey IgG antibody. The result shows that the GTX60865 does not react to monkey IgG.

Rabbit Anti-Human IgG4 antibody [RM120]. GTX60865

GTX60865 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60865 Human IgG4 antibody [RM120].

REFERENCE

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REVIEW

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Package List Price ($)
$ 339