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Rabbit Anti-Mouse IgG antibody, F(ab')2 fragment, pre-adsorbed (PE)

Rabbit Anti-Mouse IgG antibody, F(ab')2 fragment, pre-adsorbed (PE). GTX27000

Cat. No. GTX27000

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG F(ab')2

Application

 ICC/IF, FACS, Dot

Reactivity

 Mouse
Package
500 μl ($259)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:250
FACS 1:100-1:250
Dot Assay dependent
Not tested in other applications.

Product Note

Pre-adsorbed with Human serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.
From serum

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Rabbit Anti-Mouse IgG antibody, F(ab')2 fragment, pre-adsorbed (PE). GTX27000

GTX27000 Dot Image

Dot blot analysis of mouse IgG using GTX27000 Rabbit Anti-Mouse IgG antibody, F(ab')2 fragment, pre-adsorbed (PE).
Lane 1 : 100 ngLane 2 : 33.3 ng
:Lane 3 : 11.1 ng
:Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 259