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Rabbit Anti-Mouse IgG antibody (HRP)

Rabbit Anti-Mouse IgG antibody (HRP). GTX213112-01

Cat. No. GTX213112-01

Reference 26

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Mouse
Package
1 ml ($149)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Highly purified whole mouse IgG

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.
Affinity Purified IgG

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_10617557

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Mouse IgG secondary antibody , antiMouse IgG antibody , anti-Mouse IgG antibody , Rabbit antiMouse IgG HRP , Rabbit anti-Mouse IgG HRP , anti Mouse IgG antibody , Rabbit anti Mouse IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

DATA IMAGES

Rabbit Anti-Mouse IgG antibody (HRP). GTX213112-01

GTX213112-01 ELISA Image

An ELISA plate is coated with 50 μL of a 6x-His recombinant protein conjugated BSA at concentration ranged from 0.003 μg/mL to 3 μg/mL. The coated protein is detected with anti-His antibody (GTX628921) at 0.5 ng/mL. Bound antibodies were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213112-01).

REFERENCE

Application Reference

Lin ZS et al. Elife 2023; 12 EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression.
Schmidt S et al. Nat Commun 2023; 14 (1) : 7674 A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.
Kuchukulla RR et al. Pharmaceuticals (Basel) 2022; 15 (9) Novel 2,6,9-Trisubstituted Purines as Potent CDK Inhibitors Alleviating Trastuzumab-Resistance of HER2-Positive Breast Cancers.
Schmidt S et al. Nat Commun 2022; 13 (1) : 4819 Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.
Kim HJ et al. Chem Biol Interact 2022; 352 : 109782 Novel anti-adipogenic effect of CF3-allylated indole in 3T3-L1 cells.
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REVIEW

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Application Reference
Lin ZS et al. Elife 2023; 12 EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression.
Schmidt S et al. Nat Commun 2023; 14 (1):7674 A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.
Kuchukulla RR et al. Pharmaceuticals (Basel) 2022; 15 (9) Novel 2,6,9-Trisubstituted Purines as Potent CDK Inhibitors Alleviating Trastuzumab-Resistance of HER2-Positive Breast Cancers.
Schmidt S et al. Nat Commun 2022; 13 (1):4819 Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.
Kim HJ et al. Chem Biol Interact 2022; 352:109782 Novel anti-adipogenic effect of CF3-allylated indole in 3T3-L1 cells.
Chun-Hsu Pan et al. Int J Mol Sci 2021; 22 (16) Pathological Role of Phosphoglycerate Kinase 1 in Balloon Angioplasty-Induced Neointima Formation.
Cone AS et al. Theranostics 2021; 11 (17):8129-8142 Mesenchymal stem cell-derived extracellular vesicles ameliorate Alzheimer's disease-like phenotypes in a preclinical mouse model.
Pan CH et al. Evid Based Complement Alternat Med 2021; 2021:8466543 Preventive Effect and Mechanism of Crossostephium chinense Extract on Balloon Angioplasty-Induced Neointimal Hyperplasia.
Sun L et al. Sci Rep 2021; 11 (1):12477 Multiplex protein profiling method for extracellular vesicle protein detection.
Chang CH et al. Fish Physiol Biochem 2021; Hypothermal effects on expression of regucalcin, a calcium-binding protein, in the livers of seawater- and fresh water-acclimated milkfish, Chanos chanos.
Cho YH et al. J Exerc Rehabil 2021; 17 (2):96-102 Effect of treadmill exercise on pain-related Wnt/β-catenin signaling pathway in dorsal root ganglion neurons at the early phase regeneration of the injured sciatic nerve.
Lin YT et al. Comp Biochem Physiol B Biochem Mol Biol 2021; 253:110562 Differential expression of two ATPases revealed by lipid raft isolation from gills of euryhaline teleosts with different salinity preferences.
Pan CH et al. Chem Biol Interact 2020; 332:109304
Liu WQ et al. PLoS Negl Trop Dis 2020; 14 (8):e0008660 The Ras/ERK signaling pathway couples antimicrobial peptides to mediate?resistance to?dengue virus?in Aedes mosquitoes.
Morquecho-Campos P et al. Physiol Behav 2020; 226:113116 A stepwise approach investigating salivary responses upon multisensory food cues.
Sharma A et al. J Biomed Sci 2020; 27 (1):55 Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells.
Chang CH et al. Sci Rep 2019; 9 (1):18502 Changes in hypothermal stress-induced hepatic mitochondrial metabolic patterns between fresh water- and seawater-acclimated milkfish, Chanos chanos.
Pan CH et al. Aging (Albany NY) 2019; 11 (19):8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.
Morquecho-Campos P et al. Appetite 2019; 143:104399 Impact of food odors signaling specific taste qualities and macronutrient content on saliva secretion and composition.
Yang SH et al. Artif Cells Nanomed Biotechnol 2019; 47 (1):3021-3028 CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells.
Wang SH et al. PLoS One 2017; 12 (8):e0183692 Expression and potential roles of sodium-potassium ATPase and E-cadherin in human gastric adenocarcinoma.
Lenka G et al. Sci Rep 2017; 7:42573 Identification of Methylation-Driven, Differentially Expressed STXBP6 as a Novel Biomarker in Lung Adenocarcinoma.
Lin HY et al. Algal Research 2017; 23 (2017):58-65 Alkaline phosphatase promoter as an efficient driving element for exogenic recombinant in the marine diatom Phaeodactylum tricornutum.
Shih YL et al. In Vivo 2017; 31 (6):1103-1114 Fisetin Induces Apoptosis of HSC3 Human Oral Cancer Cells Through Endoplasmic Reticulum Stress and Dysfunction of Mitochondria-mediated Signaling Pathways.
Hurwitz SN et al. J Extracell Vesicles 2016; 5:31295 Nanoparticle analysis sheds budding insights into genetic drivers of extracellular vesicle biogenesis.
Yang WK et al. Comp Biochem Physiol B Biochem Mol Biol 2016; 202:23-30 Different expression patterns of renal Na+/K+-ATPase α-isoform-like proteins between tilapia and milkfish following salinity challenges.
SDS
Proclin.pdf
Package List Price ($)
$ 149