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Rabbit Anti-Mouse IgG2a antibody [RM219]

Rabbit Anti-Mouse IgG2a antibody [RM219]. GTX20293
Rabbit Anti-Mouse IgG2a antibody [RM219]. GTX20293
Rabbit Anti-Mouse IgG2a antibody [RM219]. GTX20293

Cat No. GTX20293

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM219

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ELISA, IHC

Reactivity

Mouse
Package
100 μg ($329)

APPLICATION

Application Note

ELISA: 0.005 ug/mL – 0.2 ug/mL, Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL, Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL, Western Blot (WB): 0.5 ug/mL-2 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG2a

Target Isotype

IgG2a

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

immunoglobulin heavy constant gamma 2A Antibody , Ighg2a Antibody

DATA IMAGES

Rabbit Anti-Mouse IgG2a antibody [RM219]. GTX20293

GTX20293 ELISA Image

ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coatedwith 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was usedas the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondaryantibody.

Rabbit Anti-Mouse IgG2a antibody [RM219]. GTX20293

GTX20293 ELISA Image

A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugatedanti-rabbit IgG as the secondary antibody.

Rabbit Anti-Mouse IgG2a antibody [RM219]. GTX20293

GTX20293 WB Image

Western blot of nonreduced(-) and reduced(+)mouse IgG2a, using 0.5ug/mL of RevMAb cloneRM219. This antibody reacts to nonreducedIgG2a (~150 kDa).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329