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Rabbit Anti-Mouse IgG2c antibody [RM223]

Rabbit Anti-Mouse IgG2c antibody [RM223]. GTX20294
Rabbit Anti-Mouse IgG2c antibody [RM223]. GTX20294
Rabbit Anti-Mouse IgG2c antibody [RM223]. GTX20294

Cat No. GTX20294

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM223

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ELISA, IHC

Reactivity

Mouse
Package
100 μg ($329)

APPLICATION

Application Note

ELISA: 0.01 ug/mL – 0.2 ug/mL, Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL, Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL, Western Blot (WB): 0.5 ug/mL-2 ug/mL

Specificity/Sensitivity

This antibody reacts to the Fc region of mouse IgG2c.No cross reactivity with mouse IgG1, IgG2a, IgG2b,IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG2c

Target Isotype

IgG2c

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

immunoglobulin heavy constant gamma 2C Antibody , Ighg2c Antibody

DATA IMAGES

Rabbit Anti-Mouse IgG2c antibody [RM223]. GTX20294

GTX20294 ELISA Image

A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Rabbit Anti-Mouse IgG2c antibody [RM223]. GTX20294

GTX20294 ELISA Image

ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does notreact to BalB/C IgG containing IgG2a. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.

Rabbit Anti-Mouse IgG2c antibody [RM223]. GTX20294

GTX20294 WB Image

Western blot of nonreduced(-) and reduced(+)mouse IgG2c, using 0.5ug/mL of cloneRM223. This antibody reacts to nonreduced IgG2c

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329